7K67
Structure of the PTP-like myo-inositol phosphatase from Desulfovibrio magneticus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-02-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.009, 97.320, 104.100 |
| Unit cell angles | 90.00, 92.29, 90.00 |
Refinement procedure
| Resolution | 71.070 - 1.600 |
| R-factor | 0.156 |
| Rwork | 0.156 |
| R-free | 0.16990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mmj |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.871 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 71.700 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 176115 | 8595 |
| <I/σ(I)> | 10.6 | |
| Completeness [%] | 96.2 | |
| Redundancy | 2.9 | |
| CC(1/2) | 0.996 | 0.646 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | PEG 4000 and 1000, 2-methyl-2,4-pentanediol, carboxylates, sodium acetate |
