7K5Q

Bst DNA polymerase I time-resolved structure, 8 min post dATP addition

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2019-09-27
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	88.200, 93.988, 105.724
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.920 - 2.000
R-factor	0.1949
Rwork	0.194
R-free	0.24640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6dsy
RMSD bond length	0.008
RMSD bond angle	0.866
Data reduction software	autoXDS
Data scaling software	autoXDS
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.920	2.071
High resolution limit [Å]	2.000	2.000
Rmerge	0.065	0.299
Rmeas	0.074	0.335
Rpim	0.034	0.149
Number of reflections	59144	5882
<I/σ(I)>	14.87
Completeness [%]	95.7
Redundancy	4.7
CC(1/2)	0.996	0.951

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	50% saturated ammonium sulfate, 0.1M MES, 2% MPD