7K44
SGBP-B from a complex xyloglucan utilization locus in Bacteroides uniformis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-30 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 119.864, 66.718, 57.966 |
| Unit cell angles | 90.00, 115.24, 90.00 |
Refinement procedure
| Resolution | 34.850 - 1.450 |
| R-factor | 0.1799 |
| Rwork | 0.178 |
| R-free | 0.20640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5e7g |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.784 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.850 | 1.506 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.052 | 1.515 |
| Rmeas | 0.056 | 1.660 |
| Rpim | 0.022 | 0.670 |
| Number of reflections | 72049 | 41563 |
| <I/σ(I)> | 15.92 | 0.92 |
| Completeness [%] | 99.2 | 97.85 |
| Redundancy | 6.6 | 5.9 |
| CC(1/2) | 0.999 | 0.430 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.2M magnesium chloride, 0.1M Tris pH 8.5, 25% PEG 3350 |
