7K3K
Crystal structure of dLC8 in complex with Panoramix TQT peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-07-18 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 45.639, 45.639, 202.717 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.794 - 1.415 |
| R-factor | 0.1842 |
| Rwork | 0.183 |
| R-free | 0.20060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zke |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.806 |
| Data reduction software | XDS |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 101.360 | 1.440 |
| High resolution limit [Å] | 1.415 | 1.420 |
| Rmerge | 0.069 | |
| Number of reflections | 24954 | 999 |
| <I/σ(I)> | 33.6 | |
| Completeness [%] | 99.1 | |
| Redundancy | 15.6 | |
| CC(1/2) | 0.999 | 0.946 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M NH4Ac, 0.1 M Bis-Tris pH 5.5, 17% (w/v) PEG 10000 |
