7K3K

Crystal structure of dLC8 in complex with Panoramix TQT peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-18
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 61 2 2
Unit cell lengths	45.639, 45.639, 202.717
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.794 - 1.415
R-factor	0.1842
Rwork	0.183
R-free	0.20060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3zke
RMSD bond length	0.005
RMSD bond angle	0.806
Data reduction software	XDS
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	101.360	1.440
High resolution limit [Å]	1.415	1.420
Rmerge	0.069
Number of reflections	24954	999
<I/σ(I)>	33.6
Completeness [%]	99.1
Redundancy	15.6
CC(1/2)	0.999	0.946

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M NH4Ac, 0.1 M Bis-Tris pH 5.5, 17% (w/v) PEG 10000