7K3I

Cellular retinol-binding protein 2 (CRBP2) in complex with 2-lauroylglycerol

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	90
Detector technology	PIXEL
Collection date	2019-07-18
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	37.269, 64.560, 67.029
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.499 - 1.200
R-factor	0.1847
Rwork	0.184
R-free	0.20650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7jvg
RMSD bond length	0.007
RMSD bond angle	1.035
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	64.560	1.220
High resolution limit [Å]	1.200	1.200
Rmerge	0.071	0.899
Rmeas	0.085
Rpim	0.045	0.664
Number of reflections	51014	2139
<I/σ(I)>	8.4
Completeness [%]	98.8	85.6
Redundancy	6.4
CC(1/2)	0.998	0.671

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	Tris/HCl Ph 8.0, PEG3350 20%-25%