7K2Z

Crystal structure of Pisum sativum KAI2 Apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2019-10-04
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	87.595, 71.146, 49.065
Unit cell angles	90.00, 117.62, 90.00

Refinement procedure

Resolution	43.470 - 1.610
R-factor	0.1574
Rwork	0.156
R-free	0.17960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5z9h
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.470	1.668
High resolution limit [Å]	1.610	1.610
Number of reflections	34306	3156
<I/σ(I)>	31.01
Completeness [%]	99.2
Redundancy	6.4
CC(1/2)	1.000	0.746

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.1M HEPES pH 7.5, 2.75% PEG 2000, 2.75% PEG 3350, 2.75% PEG 4000, 2.75% PEG-ME 5000