7K0X
Marsupial T cell receptor Spl_145
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-03-05 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 71.925, 87.112, 214.408 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.650 - 3.100 |
| R-factor | 0.2051 |
| Rwork | 0.203 |
| R-free | 0.25070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | unpublished |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.681 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.260 | 3.310 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rpim | 0.123 | 0.643 |
| Number of reflections | 25260 | 4496 |
| <I/σ(I)> | 7 | 2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.8 | 7.1 |
| CC(1/2) | 0.789 | 0.653 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20-25% PEG 3350, 0.2M Na Formate |
