7K06

Crystal structures and ribonuclease activity of the Flavivirus host factor ERI3 that is involved in viral RNA synthesis define the ERI subfamily of structure-specific 3-prime - 5-prime exoribonucleases

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-07
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97934
Spacegroup name	P 21 21 21
Unit cell lengths	58.417, 74.234, 96.836
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.550 - 1.950
R-factor	0.1836
Rwork	0.181
R-free	0.22260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2xri
RMSD bond length	0.003
RMSD bond angle	0.608
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.400	1.980
High resolution limit [Å]	1.950	1.950
Rmeas	0.044
Rpim	0.012
Number of reflections	31492	1497
<I/σ(I)>	58.1
Completeness [%]	99.8
Redundancy	12.5
CC(1/2)		0.795

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.8	290	25% MPEG 2K, 15% saturated KCl, 200 mM pH 7.8 imidazole / malate