7K05

Crystal structures and ribonuclease activity of the Flavivirus host factor ERI3 that is involved in viral RNA synthesis define the ERI subfamily of structure-specific 3-prime - 5-prime exoribonucleases

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.005
Spacegroup name	P 21 21 21
Unit cell lengths	58.579, 74.346, 96.513
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.250 - 1.850
R-factor	0.1734
Rwork	0.172
R-free	0.21170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2xri
RMSD bond length	0.015
RMSD bond angle	1.282
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.300	1.880
High resolution limit [Å]	1.850	1.850
Rmeas	0.088
Rpim	0.027
Number of reflections	69137	3207
<I/σ(I)>	51.7
Completeness [%]	99.5
Redundancy	9.8
CC(1/2)		0.844

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.4	290	30% MPEG 2K, 20% saturated KCl, 200 mM pH 8.4 Imidazole / Malate