7JZT
Low resolution crystal structure of Zaire Ebola virus VP40 in space group P6422
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 85 |
| Detector technology | CCD |
| Collection date | 2008-11-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97934 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 105.275, 105.275, 463.738 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.900 - 3.770 |
| R-factor | 0.3165 |
| Rwork | 0.315 |
| R-free | 0.34540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ldb |
| Data reduction software | XDS (Mar 15, 2019) |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | PHENIX (1.17.1-3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 154.586 | 4.304 |
| High resolution limit [Å] | 3.770 | 3.770 |
| Rmerge | 0.141 | 1.848 |
| Rmeas | 0.150 | 1.931 |
| Rpim | 0.050 | 0.561 |
| Number of reflections | 7524 | 376 |
| <I/σ(I)> | 12.3 | 1.8 |
| Completeness [%] | 90.8 | |
| Redundancy | 13.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.4 | 293 | 0.2 M Lithium citrate tribasic tetrahydrate, 20% w/v Polyethylene glycol 3350, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
