7JZK

Crystal structure of LAIR1 ectodomain (from MGD21) in complex with Plasmodium RIFIN (PF3D7_0401300) V2 domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	C 1 2 1
Unit cell lengths	87.118, 95.839, 71.828
Unit cell angles	90.00, 103.97, 90.00

Refinement procedure

Resolution	43.170 - 2.457
R-factor	0.2254
Rwork	0.223
R-free	0.27510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7jzi
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.490
High resolution limit [Å]	2.450	6.650	2.450
Rmerge	0.104	0.060	0.423
Rmeas	0.127	0.074	0.514
Rpim	0.072	0.043	0.288
Total number of observations	57799
Number of reflections	19253	897	970
<I/σ(I)>	6.7
Completeness [%]	92.3	83	93.9
Redundancy	3	2.6	2.7
CC(1/2)		0.992	0.855

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M NaCl, 0.1 M Tris HCl pH 8.5, 20% PEG 3350