7JYV
Crystal Structure of HLA A*2402 in complex with YFSPIRVTF, an 9-mer influenza epitope
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-08 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.952, 75.775, 89.919 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.915 - 1.510 |
| R-factor | 0.1878 |
| Rwork | 0.187 |
| R-free | 0.20640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4f7m |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.877 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 34.915 | 34.910 | 1.540 |
| High resolution limit [Å] | 1.510 | 8.270 | 1.510 |
| Rmerge | 0.042 | 0.026 | 0.448 |
| Rmeas | 0.045 | 0.028 | 0.473 |
| Rpim | 0.014 | 0.010 | 0.149 |
| Total number of observations | 2818 | 30507 | |
| Number of reflections | 64117 | 384 | 3233 |
| <I/σ(I)> | 28.4 | 58.4 | 4.4 |
| Completeness [%] | 94.0 | 77.7 | 96.2 |
| Redundancy | 9.8 | 7.3 | 9.4 |
| CC(1/2) | 1.000 | 0.999 | 0.944 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 277 | 19% PEG 3350, 0.2 MgCl2 |
