7JWR

Cellular retinol-binding protein 2 (CRBP2) in complex with 2-oleoylglycerol

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	80
Detector technology	PIXEL
Collection date	2018-03-11
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9791
Spacegroup name	P 21 21 21
Unit cell lengths	58.099, 67.380, 87.999
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.485 - 1.300
R-factor	0.135588207009
Rwork	0.134
R-free	0.17380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6bti
RMSD bond length	0.009
RMSD bond angle	0.977
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	67.380	1.320
High resolution limit [Å]	1.300	1.300
Rmerge	0.070	0.786
Rmeas	0.084	0.993
Rpim	0.046	0.597
Number of reflections	83038	3342
<I/σ(I)>	10.9	1.6
Completeness [%]	97.3	80.7
Redundancy	5.8
CC(1/2)	0.996	0.627

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	0.1 M Tris HCl, PEG 3350 22-28%