7JW2

Crystal structure of Aedes aegypti Nibbler EXO domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-08
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	39.508, 71.584, 83.031
Unit cell angles	90.00, 97.32, 90.00

Refinement procedure

Resolution	37.280 - 1.500
R-factor	0.1924
Rwork	0.192
R-free	0.20740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Drosophila Nbr EXO domain
RMSD bond length	0.006
RMSD bond angle	0.798
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.18_3855)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.280	1.554
High resolution limit [Å]	1.500	1.500
Rmerge	0.033	0.153
Rmeas	0.038	0.180
Rpim	0.017	0.092
Number of reflections	72680	6711
<I/σ(I)>	26.22	5.74
Completeness [%]	98.8	91.92
Redundancy	4.5	3.4
CC(1/2)	0.997	0.983

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M potassium formate, 20% PEG3350