7JVY

Cellular retinol-binding protein 2 (CRBP2) in complex with 2-arachidonylglyceryl ether

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	80
Detector technology	PIXEL
Collection date	2017-11-05
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9791
Spacegroup name	P 21 21 21
Unit cell lengths	57.909, 67.660, 88.809
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	53.820 - 1.300
R-factor	0.1374
Rwork	0.136
R-free	0.16580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6bti
RMSD bond length	0.009
RMSD bond angle	1.233
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.820	1.320
High resolution limit [Å]	1.300	1.300
Rmerge	0.078	0.594
Rmeas	0.094	0.741
Rpim	0.051	0.436
Number of reflections	85701	4167
<I/σ(I)>	10.6	2.1
Completeness [%]	99.3	98.7
Redundancy	5.9	5.1
CC(1/2)	0.996	0.637

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	298	0.1 M Tris/HCl, PEG 3350 25%