7JVT

Crystal structure of a lambda-186 hybrid repressor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-08-08
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.9537
Spacegroup name	H 3 2
Unit cell lengths	177.338, 177.338, 108.607
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.200 - 3.160
R-factor	0.2336
Rwork	0.228
R-free	0.28140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2fjr 3bdn
Data reduction software	XDS (March2015)
Data scaling software	Aimless (0.3.11)
Phasing software	PHASER (2.7.17)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.340	44.340	3.380
High resolution limit [Å]	3.160	8.930	3.160
Rmerge	0.135	0.033	0.738
Rmeas	0.140	0.034	0.765
Rpim	0.037	0.009	0.200
Total number of observations	165170	7050	29299
Number of reflections	11372	533	2045
<I/σ(I)>	14.6	50.8	3.1
Completeness [%]	99.8	99.1	99.4
Redundancy	14.5	13.2	14.3
CC(1/2)	0.999	0.999	0.910

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289.15	0.08 M sodium chloride, 0.012 M potassium chloride, 0.04 sodium cacodylate trihydrate pH 8.0, 30% w/v (+/-)-2-methyl-2,4-pentadiol, 0.012 M spermine tetrahydrochloride