7JVH

Crystal structure of a GH43_12 retrieved from capybara gut metagenome

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LNLS BEAMLINE W01B-MX2
Synchrotron site	LNLS
Beamline	W01B-MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-12
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.459
Spacegroup name	P 1
Unit cell lengths	107.223, 107.383, 132.533
Unit cell angles	99.03, 113.38, 108.45

Refinement procedure

Resolution	49.200 - 2.480
R-factor	0.248
Rwork	0.246
R-free	0.27830
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	1.591
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELXDE
Refinement software	PHENIX (1.8)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.200	49.204	2.630
High resolution limit [Å]	2.480	7.360	2.480
Rmerge	0.065	0.025	1.067
Rmeas	0.078	0.030	1.273
Number of reflections	166175	6399	26033
<I/σ(I)>	12.37	41.61	1.06
Completeness [%]	95.9	96.5	92.8
Redundancy	3.241	3.457	3.315
CC(1/2)	0.998	0.999	0.537

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.5	291	14% PEG 10,000, 1% dioxane, 0.1 M amonium acetate, 0.1 M BIS-TRIS pH 5.5