7JVE

Crystal structure of Salmonella enterica Typhimurium BcfH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-11
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	103.012, 133.731, 146.750
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.650 - 2.310
R-factor	0.1686
Rwork	0.167
R-free	0.20850
Structure solution method	MAD
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	Auto-Rickshaw
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.340
High resolution limit [Å]	2.300	6.240	2.300
Rmerge	0.072	0.034	0.585
Rmeas	0.077	0.036	0.638
Rpim	0.025	0.010	0.245
Total number of observations	763336
Number of reflections	88024	4737	4177
<I/σ(I)>	13
Completeness [%]	99.1	99.6	94.7
Redundancy	8.7	11.3	6.3
CC(1/2)		0.999	0.805

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6	293	0.2 M MgCl2, 0.1 mM Bis Tris propane, pH 6.0, 20% PEG 3350