7JUQ

Crystal Structure of KSR2:MEK1 in complex with ADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	80
Detector technology	CCD
Collection date	2018-08-23
Detector	RAYONIX MX-300
Wavelength(s)	1
Spacegroup name	P 61 2 2
Unit cell lengths	139.000, 139.000, 222.000
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.498 - 3.220
R-factor	0.2556
Rwork	0.254
R-free	0.28800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2y4i
RMSD bond length	0.004
RMSD bond angle	0.994
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.14rc1_3177))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.300
High resolution limit [Å]	3.220	3.220
Number of reflections	21251	1543
<I/σ(I)>	20.86
Completeness [%]	99.9
Redundancy	11.98
CC(1/2)	0.999	0.514

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG 2000, MES, pH 6.5, Magnesium acetate