7JUD

C-type carbohydrate-recognition domain 4 of the mannose receptor complexed with methyl-mannoside

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 1
Unit cell lengths	30.137, 37.836, 55.065
Unit cell angles	94.08, 99.63, 104.98

Refinement procedure

Resolution	31.930 - 1.400
R-factor	0.1495
Rwork	0.148
R-free	0.18000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	IEGG
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	36.280	36.280	1.420
High resolution limit [Å]	1.400	7.640	1.400
Rmerge	0.109	0.118	0.234
Rmeas	0.118	0.126	0.254
Rpim	0.043	0.045	0.096
Total number of observations		2139	11971
Number of reflections	44495	273	1918
<I/σ(I)>	9	15.4	3.6
Completeness [%]	97.2	98.9	85.2
Redundancy	6.9	7.8	6.2
CC(1/2)	0.997	0.993	0.983

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	Protein solution: 6.0mg/ml protein in 5mM CaCl2, 10mM Tris, pH 8.0, 25mM NaCl, and 50mM Me-Man Reservoir solution: 2.5% Peg 3.35K or 10% Peg 400, 0.1M Mes pH=6.0