7JT9

Fgr SH3 domain crystal structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	I 2 2 2
Unit cell lengths	35.553, 53.287, 67.483
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.740 - 1.930
R-factor	0.1894
Rwork	0.186
R-free	0.21720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2hda
Data scaling software	HKL-2000
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.960
High resolution limit [Å]	1.930	1.930
Rmerge	0.092	0.468
Number of reflections	4945	227
<I/σ(I)>	15.875
Completeness [%]	97.5
Redundancy	4.6
CC(1/2)	1.000	0.778

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	0.1 M Sodium citrate tribasic, 0.1 M citric acid, 40% (v/v) PEG 600