7JSR

Crystal structure of the large glutamate dehydrogenase composed of 180 kDa subunits from Mycobacterium smegmatis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-02-13
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.99187
Spacegroup name	C 2 2 21
Unit cell lengths	151.562, 253.537, 399.721
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.980 - 6.270
R-factor	0.2291
Rwork	0.226
R-free	0.28430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Multiple
RMSD bond length	0.006
RMSD bond angle	1.113
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.980	7.010
High resolution limit [Å]	6.270	6.270
Rmerge	0.055	0.778
Rmeas	0.062	0.871
Rpim	0.028	0.387
Number of reflections	34034	9720
<I/σ(I)>	10.4	1
Completeness [%]	98.4	100
Redundancy	4.9	4.9
CC(1/2)	0.999	0.867

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		277	100 mM sodium cacodylate pH 5.8, 12% v/v glycerol, 1.25 M ammonium sulfate