7JRV
Phospholipase D engineered mutant bound to phosphatidic acid (30 minute soak)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
Synchrotron site | Australian Synchrotron |
Beamline | MX1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-03-20 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.953700 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 59.575, 84.363, 98.965 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.660 - 2.420 |
Rwork | 0.181 |
R-free | 0.23700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ze4 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.995 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.16) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 64.200 | 2.510 |
High resolution limit [Å] | 2.420 | 2.420 |
Rmerge | 0.140 | 0.660 |
Number of reflections | 19588 | 1905 |
<I/σ(I)> | 13.5 | |
Completeness [%] | 99.7 | |
Redundancy | 7.2 | |
CC(1/2) | 0.997 | 0.928 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6 | 283 | 15% PEG6000, 100 mM MES, pH 6.0 |