7JRR

Crystal structures of artificially designed homomeric RNA nanoarchitectures

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-04-11
Detector	PSI PILATUS 6M
Wavelength(s)	0.97918
Spacegroup name	P 43 21 2
Unit cell lengths	40.219, 40.219, 202.430
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.163 - 2.160
R-factor	0.2295
Rwork	0.226
R-free	0.25980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ID: 2NOK
RMSD bond length	0.005
RMSD bond angle	1.237
Data reduction software	HKL-2000 (718)
Data scaling software	HKL-2000 (718)
Phasing software	PHENIX (1.11.1_2575)
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.220	2.150
High resolution limit [Å]	2.088	2.090
Rmerge		1.052
Number of reflections	10347	614
<I/σ(I)>	23.3	0.9
Completeness [%]	96.8	77.7
Redundancy	7.1	2.4
CC(1/2)	0.999	0.300

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	295	50 mM HEPES pH 7.0, 20 of mM KCl, 5 mM of MnCl2, 35 % (v/v) MPD