7JRL

The structure of CBM51-2 in complex with GlcNAc and INT domains from Clostridium perfringens ZmpB

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-04-11
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91966
Spacegroup name	C 1 2 1
Unit cell lengths	150.120, 51.360, 78.190
Unit cell angles	90.00, 115.47, 90.00

Refinement procedure

Resolution	38.930 - 1.500
R-factor	0.1839
Rwork	0.183
R-free	0.19530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vmg
RMSD bond length	0.005
RMSD bond angle	1.229
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.860	1.580
High resolution limit [Å]	1.500	1.500
Rmerge	0.082	0.581
Rpim	0.040	0.283
Number of reflections	86269	12539
<I/σ(I)>	13.2	3.6
Completeness [%]	100.0	100
Redundancy	5	5
CC(1/2)	0.998	0.735

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	0.15 M sodium phosphate monobasic, 18% PEG3350, 0.1 M Tris-HCl