7JRK
The Structure of BamE from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-24 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 31 |
| Unit cell lengths | 59.402, 59.402, 62.104 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.700 - 1.710 |
| R-factor | 0.2432 |
| Rwork | 0.242 |
| R-free | 0.25940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5wam |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.686 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.730 |
| High resolution limit [Å] | 1.700 | 4.610 | 1.700 |
| Rmerge | 0.107 | 0.050 | 2.601 |
| Rmeas | 0.113 | 0.053 | 2.750 |
| Rpim | 0.037 | 0.018 | 0.889 |
| Total number of observations | 253661 | ||
| Number of reflections | 26527 | 1330 | 1356 |
| <I/σ(I)> | 7.4 | ||
| Completeness [%] | 99.9 | 99.6 | 100 |
| Redundancy | 9.6 | 9.6 | 9.5 |
| CC(1/2) | 0.998 | 0.726 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | Imidazole, sodium chloride |
