7JRK

The Structure of BamE from Pseudomonas aeruginosa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0332
Spacegroup name	P 31
Unit cell lengths	59.402, 59.402, 62.104
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.700 - 1.710
R-factor	0.2432
Rwork	0.242
R-free	0.25940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5wam
RMSD bond length	0.004
RMSD bond angle	0.686
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.730
High resolution limit [Å]	1.700	4.610	1.700
Rmerge	0.107	0.050	2.601
Rmeas	0.113	0.053	2.750
Rpim	0.037	0.018	0.889
Total number of observations	253661
Number of reflections	26527	1330	1356
<I/σ(I)>	7.4
Completeness [%]	99.9	99.6	100
Redundancy	9.6	9.6	9.5
CC(1/2)		0.998	0.726

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	Imidazole, sodium chloride