7JRI
High-resolution Crystal Structures of Transient Intermediates in the Phytochrome Photocycle, 33 ms structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | FREE ELECTRON LASER |
| Source details | SACLA BEAMLINE BL2 |
| Synchrotron site | SACLA |
| Beamline | BL2 |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2019-06-18 |
| Detector | MPCCD |
| Wavelength(s) | 1.38 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 83.690, 83.400, 86.870 |
| Unit cell angles | 90.00, 107.63, 90.00 |
Refinement procedure
| Resolution | 24.330 - 2.400 |
| R-factor | 0.29435 |
| Rwork | 0.292 |
| R-free | 0.33349 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ptq |
| RMSD bond length | 0.013 |
| RMSD bond angle | 2.120 |
| Data reduction software | CrystFEL |
| Data scaling software | CrystFEL |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 68919 | 2561 |
| <I/σ(I)> | 7.6 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 449.4 | 203 |
| CC(1/2) | 0.994 | 0.310 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 293 | 0.17 M Ammonium acetate, 0.085 M Sodium citrate tribasic dihydrate pH 5.6, 25.5% w/v Polyethylene glycol 4000, 15% v/v Glycerol (cryo-screen solution) and 3 % w/v Benzamidine Hydrochloride mixed with 60 mg/mL protein (3:2) protein to mother liquor ratio |
