7JR5
Real Time Reaction Intermediates in Stigmatella Bacteriophytochrome P2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | FREE ELECTRON LASER |
| Source details | SACLA BEAMLINE BL2 |
| Synchrotron site | SACLA |
| Beamline | BL2 |
| Temperature [K] | 289 |
| Detector technology | CCD |
| Collection date | 2018-06-18 |
| Detector | MPCCD |
| Wavelength(s) | 1.38 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 83.690, 83.400, 86.870 |
| Unit cell angles | 90.00, 107.63, 90.00 |
Refinement procedure
| Resolution | 19.970 - 2.400 |
| R-factor | 0.31412 |
| Rwork | 0.313 |
| R-free | 0.34146 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 6ptq |
| RMSD bond length | 0.010 |
| RMSD bond angle | 2.211 |
| Data reduction software | CrystFEL |
| Data scaling software | CrystFEL |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 38486 | 3035 |
| <I/σ(I)> | 15 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 543 | 158 |
| CC(1/2) | 0.960 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 7 | 289 | SaBphP2 (60 mg/mL) was mixed 3:2 with a solution containing 0.17 M Ammonium-acetate, 85 mmol/L sodium citrate, pH 6.5, 25.5 % PEG 4000, 15% glycerol, 3 % benzamidine hydrochloride. |
