7JQZ

Crystal structure of Cfl2 wild-type from Burkholderia cenocepacia

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL14-1
Synchrotron site	SSRL
Beamline	BL14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-12-03
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	1.18076
Spacegroup name	P 21 21 2
Unit cell lengths	182.734, 210.471, 86.956
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.540 - 2.200
R-factor	0.1863
Rwork	0.185
R-free	0.21650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3kd2
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.540	2.330
High resolution limit [Å]	2.200	2.200
Rmeas	0.097	0.841
Number of reflections	170389	27173
<I/σ(I)>	16.6	2.45
Completeness [%]	99.9
Redundancy	7.5
CC(1/2)	0.999	0.822

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	784 mM sodium thiocyanate, 12% PEG 3350