7JQX

Crystal structure of Cfl1 wild-type from Burkholderia cenocepacia

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-08-12
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9793
Spacegroup name	I 4 2 2
Unit cell lengths	132.150, 132.150, 341.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.630 - 2.200
R-factor	0.1817
Rwork	0.180
R-free	0.21230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3kd2
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.630	2.330
High resolution limit [Å]	2.200	2.200
Rmeas	0.178
Number of reflections	76696	11911
<I/σ(I)>	11.71
Completeness [%]	99.5
Redundancy	13.3
CC(1/2)	0.998	0.794

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	750 mM potassium sodium tartrate, 100 mM HEPES pH 8.1