7JQP

The Phi-28 gp11 DNA packaging Motor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2017-06-07
Detector	RAYONIX MX-300
Wavelength(s)	0.97872
Spacegroup name	P 43 21 2
Unit cell lengths	110.900, 110.900, 351.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.130 - 2.890
R-factor	0.2282
Rwork	0.226
R-free	0.27550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7jq6
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER (2.7.18)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	70.000	70.000	2.950
High resolution limit [Å]	2.890	7.870	2.890
Rmerge	0.185	0.106	2.111
Rmeas	0.194	0.111	2.362
Rpim	0.057	0.033	1.027
Number of reflections	72712	2751	2220
<I/σ(I)>	5.8	12.1	0.6
Completeness [%]	98.9	97.8	89.8
Redundancy	10.9	10.9	4.8
CC(1/2)	0.982	0.989	0.302

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.3	277	1.0 M trisodium citrate; 0.1 M tris(hydroxymethyl)aminomethane (Tris), pH 8.3