7JQE
Structure of an extracellular fragment of EsaA from Streptococcus gallolyticus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-02-14 |
| Detector | DECTRIS PILATUS3 X 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 74.466, 248.235, 81.095 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.370 - 2.400 |
| R-factor | 0.2169 |
| Rwork | 0.215 |
| R-free | 0.26160 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.427 |
| Data reduction software | DIALS |
| Data scaling software | DIALS |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.300 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.136 | 0.954 |
| Rpim | 0.069 | 0.644 |
| Number of reflections | 29568 | 1310 |
| <I/σ(I)> | 6.1 | 1 |
| Completeness [%] | 98.7 | 89.3 |
| Redundancy | 4.7 | |
| CC(1/2) | 0.990 | 0.513 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.2M MgCl2, 0.1M Tris-HCl pH 7.0, 10% (w/v) PEG 8000. |
