7JQ7

The Phi-28 gp11 DNA packaging Motor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2016-08-17
Detector	RAYONIX MX-300
Wavelength(s)	0.97872
Spacegroup name	P 43 21 2
Unit cell lengths	112.200, 112.200, 354.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.689 - 2.895
R-factor	0.237
Rwork	0.235
R-free	0.28370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7jq6
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER (2.7.18)
Refinement software	PHENIX (1.13rc2_2986)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.050
High resolution limit [Å]	2.895	8.130	2.895
Rmerge	0.167	0.047	2.200
Rmeas	0.172	0.049	2.332
Rpim	0.041	0.012	0.761
Number of reflections	78650	2605	2285
<I/σ(I)>	19.2	46.7	1
Completeness [%]	99.9	99.4	99.9
Redundancy	17.3	16.3	9.2
CC(1/2)	0.985	0.996	0.330

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.1	292	1.0 M trisodium citrate; 0.1 M tris(hydroxymethyl)aminomethane (Tris), pH 8.1