7JQ6

The Phi-28 gp11 DNA packaging Motor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2017-10-15
Detector	RAYONIX MX-300
Wavelength(s)	0.97872
Spacegroup name	P 32 2 1
Unit cell lengths	135.000, 135.000, 276.700
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.382 - 2.900
R-factor	0.2427
Rwork	0.241
R-free	0.27410
Structure solution method	SAD
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELX
Refinement software	PHENIX (1.13rc2_2986)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.850
High resolution limit [Å]	2.800	2.800
Rmerge	0.183
Rpim	0.028	1.450
Number of reflections	82234	1138
<I/σ(I)>	16.9	0.495
Completeness [%]	85.2	31.8
Redundancy	42.7	12.3
CC(1/2)		0.518

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.7	293	1.6 M ammonium sulfate, 0.1 M trisodium citrate, pH 5.7