7JPA
Self-assembly of a 3D DNA crystal lattice (4x6 duplex version) containing the J5 immobile Holliday junction
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-02-15 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1 |
Spacegroup name | P 32 |
Unit cell lengths | 68.167, 68.167, 53.235 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 34.084 - 3.164 |
R-factor | 0.2025 |
Rwork | 0.201 |
R-free | 0.21620 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5vy6 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.244 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.200 |
High resolution limit [Å] | 3.150 | 8.540 | 3.150 |
Rmerge | 0.103 | 0.083 | 0.299 |
Rmeas | 0.109 | 0.088 | 0.321 |
Rpim | 0.036 | 0.029 | 0.113 |
Number of reflections | 3637 | 246 | 93 |
<I/σ(I)> | 6.2 | ||
Completeness [%] | 73.2 | 98 | 36.3 |
Redundancy | 8.2 | 10.1 | 6.2 |
CC(1/2) | 1.000 | 0.995 | 0.928 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.5 mL of 0.05 M Cacodylate pH 6.0 with 200 mM Ca(CH3COO)2, 1.0 mM CoH18N6, and 2.0 M LiCl was added to the reservoir with 2 uL added to the drop containing 4 uL of DNA stock |