7JOW
Crystal structure of BbKI complexed with Human Kallikrein 4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-12-14 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 100.495, 100.495, 167.298 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.010 - 1.910 |
| R-factor | 0.1753 |
| Rwork | 0.174 |
| R-free | 0.20750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7jod |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.276 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.010 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 39402 | 1906 |
| <I/σ(I)> | 43.34 | 1.83 |
| Completeness [%] | 100.0 | 99 |
| Redundancy | 16.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 2.4M Ammounium Sulfate, 2% PEG400 10mM CdCl2 |
