Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-19 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.035 |
| Spacegroup name | P 1 |
| Unit cell lengths | 119.242, 119.135, 382.903 |
| Unit cell angles | 89.84, 90.00, 119.96 |
Refinement procedure
| Resolution | 10.000 - 3.950 |
| R-factor | 0.261 |
| Rwork | 0.259 |
| R-free | 0.29140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6w7t |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.775 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2-3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 4.130 |
| High resolution limit [Å] | 3.950 | 3.950 |
| Rpim | 0.205 | |
| Number of reflections | 150584 | 19187 |
| <I/σ(I)> | 3.2 | 1.2 |
| Completeness [%] | 94.0 | 93 |
| Redundancy | 1.6 | |
| CC(1/2) | 0.350 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | 0.1 M succinic acid, 15% w/v PEG 3350 |
