7JOG
Self-assembly of a 3D DNA crystal lattice (4x6 duplex version) containing the J33 immobile Holliday junction
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-11-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 32 |
| Unit cell lengths | 68.389, 68.389, 60.348 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.195 - 3.101 |
| R-factor | 0.2381 |
| Rwork | 0.236 |
| R-free | 0.27340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5vy6 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.749 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.150 |
| High resolution limit [Å] | 3.100 | 8.400 | 3.100 |
| Rmerge | 0.064 | 0.044 | 0.385 |
| Rmeas | 0.071 | 0.048 | 0.433 |
| Rpim | 0.030 | 0.020 | 0.194 |
| Total number of observations | 23644 | ||
| Number of reflections | 4538 | 280 | 110 |
| <I/σ(I)> | 9.1 | ||
| Completeness [%] | 79.0 | 97.9 | 38.5 |
| Redundancy | 5.2 | 5.8 | 3.9 |
| CC(1/2) | 0.996 | 0.920 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.5 mL of 0.05 M Na succinate pH 5.5 with 10 mM MgCl2, 2.0 mM CoH18N6, and 10% isopropanol was added to the reservoir with 2 uL added to the drop containing 4 uL of DNA stock |
