7JNF

The structure of CBM32-1 and CBM32-2 domains from Clostridium perfringens ZmpB in complex with GalNAc

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-04-11
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91966
Spacegroup name	P 43 21 2
Unit cell lengths	89.740, 89.740, 187.490
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.570 - 2.100
R-factor	0.2325
Rwork	0.231
R-free	0.25630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7jnb
RMSD bond length	0.002
RMSD bond angle	0.689
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.870	2.210
High resolution limit [Å]	2.100	2.100
Rmerge	0.120	0.602
Rpim	0.048	0.242
Number of reflections	45632	6542
<I/σ(I)>	10.1	2.9
Completeness [%]	100.0	100
Redundancy	7.2	7.1
CC(1/2)	0.995	0.773

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	1.6 M tribasic ammonium citrate, 3% glycerol