7JNB

The structure of CBM32-1 and CBM32-2 domains from Clostridium perfringens ZmpB in complex with GalNAc

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-04-11
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91966
Spacegroup name	P 43 21 2
Unit cell lengths	89.610, 89.610, 185.870
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.510 - 2.000
R-factor	0.2105
Rwork	0.209
R-free	0.23490
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	1.020
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.510	2.110
High resolution limit [Å]	2.000	2.000
Rmerge	0.114	1.017
Rpim	0.038	0.341
Number of reflections	50493	7126
<I/σ(I)>	11.4	2.2
Completeness [%]	97.2	95.7
Redundancy	8.6	8.4
CC(1/2)	0.998	0.605

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	1.6 M tribasic ammonium citrate, 3% glycerol