7JNB
The structure of CBM32-1 and CBM32-2 domains from Clostridium perfringens ZmpB in complex with GalNAc
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-11 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.91966 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 89.610, 89.610, 185.870 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.510 - 2.000 |
| R-factor | 0.2105 |
| Rwork | 0.209 |
| R-free | 0.23490 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.020 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.510 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.114 | 1.017 |
| Rpim | 0.038 | 0.341 |
| Number of reflections | 50493 | 7126 |
| <I/σ(I)> | 11.4 | 2.2 |
| Completeness [%] | 97.2 | 95.7 |
| Redundancy | 8.6 | 8.4 |
| CC(1/2) | 0.998 | 0.605 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 1.6 M tribasic ammonium citrate, 3% glycerol |
