7JMU
Hen egg-white lysozyme with ionic liquid ethylammonium nitrate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-10-16 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.953646 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 27.585, 62.637, 59.645 |
| Unit cell angles | 90.00, 90.54, 90.00 |
Refinement procedure
| Resolution | 29.820 - 1.200 |
| R-factor | 0.149 |
| Rwork | 0.147 |
| R-free | 0.18270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dpw |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.236 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 43.190 | 43.190 | 1.220 |
| High resolution limit [Å] | 1.200 | 6.570 | 1.200 |
| Rmerge | 0.046 | 0.029 | 0.345 |
| Rmeas | 0.049 | 0.031 | 0.378 |
| Rpim | 0.019 | 0.012 | 0.152 |
| Total number of observations | 417805 | 2709 | 15754 |
| Number of reflections | 60488 | 406 | 2631 |
| <I/σ(I)> | 19.5 | 54 | 3.9 |
| Completeness [%] | 95.5 | 99.1 | 85.2 |
| Redundancy | 6.9 | 6.7 | 6 |
| CC(1/2) | 0.999 | 0.999 | 0.938 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 8 | 298.15 | 5.7% w/v ethylammonium nitrate |
