7JM4

IRF Transcription Factor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2019-04-17
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9537
Spacegroup name	P 31 2 1
Unit cell lengths	117.802, 117.802, 154.579
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.440 - 2.950
R-factor	0.1859
Rwork	0.185
R-free	0.20550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2irf
RMSD bond length	0.003
RMSD bond angle	0.621
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.440	3.055
High resolution limit [Å]	2.950	2.950
Rmerge	0.105	1.251
Rmeas	0.111
Rpim		1.318
Number of reflections	26659	2492
<I/σ(I)>	19.23
Completeness [%]	99.0	100
Redundancy	11.1
CC(1/2)	0.999	0.692

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	10 % PEG 4000, 0.1 M Na acetate pH 4.6