7JM0

Crystal structure of aminoglycoside resistance enzyme ApmA, apoenzyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2019-01-14
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	68.544, 76.916, 96.572
Unit cell angles	90.00, 102.12, 90.00

Refinement procedure

Resolution	25.000 - 2.080
R-factor	0.1894
Rwork	0.188
R-free	0.22230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7jm1
RMSD bond length	0.003
RMSD bond angle	0.526
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.15_3448)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.120
High resolution limit [Å]	2.080	2.080
Rmerge	0.109	0.451
Rpim	0.075	0.297
Number of reflections	58261	2886
<I/σ(I)>	19.39	2.08
Completeness [%]	98.7
Redundancy	3.2
CC(1/2)		0.771

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	5% 2-Propanol, 0.1 M Citric acid pH 3.5, 6% PEG 20K cryoprotectant 25% ethylene glycol