7JLZ

Crystal structure of 30S ribosomal A1408 methyltransferase from an uncultured bacterium (UncKam)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-24
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1
Spacegroup name	C 2 2 21
Unit cell lengths	50.370, 66.330, 133.010
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.110 - 1.640
R-factor	0.1982
Rwork	0.196
R-free	0.22420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Swiss Model using 3MTE as template
RMSD bond length	0.006
RMSD bond angle	0.803
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.115	1.699
High resolution limit [Å]	1.640	1.640
Rmerge	0.082
Rmeas	0.087
Rpim	0.026	0.660
Number of reflections	27763	2748
<I/σ(I)>	13.55
Completeness [%]	99.9	99.78
Redundancy	10.9	11.3
CC(1/2)	0.998	0.655

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	20-28% PEG 8000, 0.1M MES pH= 5.5-7.0, 0.2M Ammonium Sulfate