7JLY
Crystal Structure of RNA Binding Protein PG1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2015-07-29 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 40.128, 40.128, 83.676 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.752 - 1.730 |
| R-factor | 0.1794 |
| Rwork | 0.178 |
| R-free | 0.21130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zka |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.969 |
| Data reduction software | d*TREK (9.9.8.6 W9RSSI) |
| Data scaling software | d*TREK (9.9.8.6 W9RSSI) |
| Phasing software | PHASER (2.8.2) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 34.760 | 2.350 | 1.790 |
| High resolution limit [Å] | 1.730 | 2.180 | 1.730 |
| Rmerge | 0.074 | 0.120 | 0.349 |
| Rmeas | 0.076 | 0.123 | 0.362 |
| Number of reflections | 8483 | 843 | 776 |
| <I/σ(I)> | 22.5 | 18.8 | 6.7 |
| Completeness [%] | 98.4 | 99.5 | 93.7 |
| Redundancy | 17.57 | 17.34 | 14.59 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 277 | 0.1M MES, pH6.0, 22% PEG 8000, 0.2M Ca Acetate |
