7JL6
Heme binding to SrrB PAS domain plays a role in redox regulation of S. aureus SrrAB two-component system
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-01-30 |
| Detector | NOIR-1 |
| Wavelength(s) | 1.00003 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 84.072, 65.140, 37.513 |
| Unit cell angles | 90.00, 91.11, 90.00 |
Refinement procedure
| Resolution | 51.490 - 2.100 |
| R-factor | 0.19444 |
| Rwork | 0.192 |
| R-free | 0.23437 |
| Structure solution method | AB INITIO PHASING |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.305 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.490 | 51.490 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmeas | 0.235 | 0.235 |
| Number of reflections | 10615 | 1041 |
| <I/σ(I)> | 3.28 | |
| Completeness [%] | 94.0 | 94.13 |
| Redundancy | 10.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 2.4M sodium malonate pH 7.0 |
