7JL5

Crystal structure of human NEIL3 tandem zinc finger GRF domains

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	105
Detector technology	CCD
Collection date	2016-08-05
Detector	MARRESEARCH
Wavelength(s)	1.0000
Spacegroup name	I 4
Unit cell lengths	93.494, 93.494, 63.646
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.950 - 2.600
R-factor	0.2307
Rwork	0.227
R-free	0.26610
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.675
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.640
High resolution limit [Å]	2.600	2.600
Rmerge	0.038	0.656
Rmeas	0.043	0.742
Rpim	0.020	0.344
Number of reflections	13020	435
<I/σ(I)>	13.4
Completeness [%]	99.8	100
Redundancy	4.5	4.6
CC(1/2)		0.840

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.5	293	PEG 6000