7JKB
2xVH Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-24 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 70.480, 195.000, 91.490 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.750 - 2.550 |
| R-factor | 0.2578 |
| Rwork | 0.254 |
| R-free | 0.29160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | modeled Fab |
| Data reduction software | xia2 |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3211) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.750 | 48.750 | 2.620 |
| High resolution limit [Å] | 2.550 | 11.400 | 2.550 |
| Rmerge | 0.054 | 0.028 | 1.044 |
| Number of reflections | 20965 | 276 | 2042 |
| <I/σ(I)> | 18.8 | ||
| Completeness [%] | 99.9 | 98.6 | 99.9 |
| Redundancy | 6.6 | 5.2 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 1M lithium chloride, 0.1M citric acid pH 4.0 |
