7JJZ
Self-assembly of a 3D DNA crystal lattice (4x6 scramble junction version) containing the J2 immobile Holliday junction
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-09-15 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 32 |
| Unit cell lengths | 68.272, 68.272, 55.786 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.563 - 3.068 |
| R-factor | 0.2408 |
| Rwork | 0.238 |
| R-free | 0.26460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6xna |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.730 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.100 |
| High resolution limit [Å] | 3.050 | 8.270 | 3.050 |
| Rmerge | 0.109 | 0.090 | 0.833 |
| Rmeas | 0.115 | 0.094 | 0.892 |
| Rpim | 0.037 | 0.029 | 0.307 |
| Number of reflections | 4231 | 281 | 135 |
| <I/σ(I)> | 4.5 | ||
| Completeness [%] | 77.7 | 100 | 46.1 |
| Redundancy | 8.6 | 9.7 | 7.5 |
| CC(1/2) | 0.981 | 0.998 | 0.785 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.5 mL of 0.05 M Cacodylate pH 6.0 with 20 mM MgCl2, 1.0 mM spermine, and15% ethanol was added to the reservoir with 2 uL added to the drop containing 4 uL of DNA stock |
