7JJV
Crystal waters on the nine polyproline type II helical bundle springtail antifreeze protein from Granisotoma rainieri match the ice lattice
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-02-05 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 30.489, 64.214, 86.267 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.171 - 1.210 |
Rwork | 0.149 |
R-free | 0.18040 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2pne |
RMSD bond length | 0.017 |
RMSD bond angle | 2.115 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.171 | 1.230 |
High resolution limit [Å] | 1.210 | 1.210 |
Rmerge | 0.064 | 0.925 |
Rmeas | 0.067 | 0.963 |
Rpim | 0.019 | 0.266 |
Number of reflections | 52608 | 2579 |
<I/σ(I)> | 16.5 | 2.5 |
Completeness [%] | 99.9 | 99.7 |
Redundancy | 12.4 | 12.7 |
CC(1/2) | 0.999 | 0.923 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 277.15 | 0.2 M lithium nitrate, 2.2 M ammonium sulfate |